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Postal address
René Brons
Core Facility Cytometry
CRP-Santé
84, Val Fleuri
L-1536 Luxembourg

Delivery address
René Brons
Core Facility Cytometry
CRP-Santé
CHL Maternité Basement Rm 7
120, route d’Arlon
L-1150 Luxembourg
Tel.: +352 26970-308
Fax: +352 26970-397
Email : rene.brons@crp-sante.lu

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Flow Cytometry

The Flow Cytometry Competence Center (CFC) within the Luxembourg Biomedical Research Resources (LBR2) at CRP-Santé is a shared core resource facility that is available to assist researchers with flow cytometry experimental design, data acquisition, cell sorting, and data analysis. The CFC is an open facility accessible to all scientists at CRP-Santé as well as external researchers.

The CFC is equipped with a state-of-the art FACSAria cell sorter and a FACSCanto analyzer. The CFC is available to provide the following expertise (additional flow cytometry techniques not listed here may be implemented upon request):

  • Multicolor analysis, with a maximum of 17 parameters
  • Cell sorting, including single cell sort with variable receptors
  • Side population, apoptosis, and calcium flux detection
  • Cytometry Beads Array (CBA) immunoassay
  • Cell cycle studies
  • Assistance with experimental design and data processing

Additional flowcytometry techniques can be implemented.

For more information contact René Brons .

CFC-Services

The Flow Cytometry core laboratory was developed in 2004 under the direction of Rene Brons. With over 38 years of experience in flow cytometry, Mr. Brons has continually updated the Flow Cytometry facility with state-of-the-art equipment. In 2012 a collaboration is established between the CFC and the Nancytometrie platform in Nancy (Fr) which favours the use of the ImageStream. Mr. Brons has also led the laboratory with over 35 publications in internationally recognized scientific journals.

The Core Facility Cytometry (CFC) is the centralized flowcytometry service of the Centre Recherche Public de la Santé (CRP-Santé) in Luxembourg. Established in 2005, the CFC assists researchers with flowcytometry experimental design, data acquisition, cell sorting  and data analysis. Currently the CFC is equipped with a FACSAria cell sorter and a FACSCanto analyser. The CFC is an open facility accessible for personnel from CRP-Santé as well as external researchers.
For more information contact René Brons .

CFC-Presentation

FACSCanto

FACSCanto

The FacsCanto is a 6 color analyzer, suitable for running carousels for upto 40 samples. This flowcytometer is relative easy to operate. Use the “Canto multicolor scheme” for choosing the correct fluorochromes. Alternative fluorchromes might be usefull. Check the FACSCanto standard configuration and the excitation and emission spectra of the alternative fluorchrome for compatibility.  Additional filters and lenses are available at the CFC for a custom configuration. Contact René Brons  for further information.

FACSAria SORP

FACSAria-SORP

The FACSAria SORP is a 4, laser,14 color cell sorter, capable of generating 4 sort streams.  The collection can be done in various tubes or plates. The complexity of the Aria requires a skilled operator for use. Use the “Aria multicolor scheme” for choosing the correct fluorochromes. Alternative fluorchromes might be useful. Check the Aria standard configuration and the excitation and emission spectra of the alternative fluorchrome for compatibility.  Additional filters and lenses are available at the CFC for a custom configuration. Contact René Brons for further information

5 Analysis stations

5_Analysis_stations

Equipment

Current Team Members :

René Brons BS  

Function : Specialist Flow Cytometry

Phone : +352 26970-308

Fax : +352 26970-397

Email : rene.brons@crp-sante.lu