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Postal address
René Brons
Core Facility Cytometry
CRP-Santé
84, Val Fleuri
L-1536 Luxembourg

Delivery address
René Brons
Core Facility Cytometry
CRP-Santé
CHL Maternité Basement Rm 7
120, route d’Arlon
L-1150 Luxembourg
Tel.: +352 26970-308
Fax: +352 26970-397
Email : rene.brons@crp-sante.lu

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Flow Cytometry

The Core Facility Cytometry (CFC) is the centralized flowcytometry service of the Centre Recherche Public de la Santé (CRP-Santé) in Luxembourg. Established in 2005, the CFC assists researchers with flowcytometry experimental design, data acquisition, cell sorting  and data analysis. Currently the CFC is equipped with a FACSAria cell sorter and a FACSCanto analyser. The CFC is an open facility accessible for personnel from CRP-Santé as well as external researchers.
For more information contact René Brons .

CFC-Presentation

Over the past years the CFC acquired expertise in the following fields:

  • Multicolour analysis, with a maximum of 16 parameters
  • Cell Sorting
  • Side Population detection
  • Single cell sort in variable receptors
  • Cytometry Beads Array (CBA)
  • Cell Cycle studies
  • Apoptosis detection
  • Calcium Flux detection
  • Telomere quantification
  • Assistance in experimental design
  • Assistance in data processing

Additional flowcytometry techniques can be implemented.

For more information contact René Brons .

CFC-Services

FACSCanto

FACSCanto

The FacsCanto is a 6 color analyzer, suitable for running carousels for upto 40 samples. This flowcytometer is relative easy to operate. Use the “Canto multicolor scheme” for choosing the correct fluorochromes. Alternative fluorchromes might be usefull. Check the FACSCanto standard configuration and the excitation and emission spectra of the alternative fluorchrome for compatibility.  Additional filters and lenses are available at the CFC for a custom configuration. Contact René Brons  for further information.

FACSAria SORP

FACSAria-SORP

The FACSAria SORP is a 4, laser,14 color cell sorter, capable of generating 4 sort streams.  The collection can be done in various tubes or plates. The complexity of the Aria requires a skilled operator for use. Use the “Aria multicolor scheme” for choosing the correct fluorochromes. Alternative fluorchromes might be useful. Check the Aria standard configuration and the excitation and emission spectra of the alternative fluorchrome for compatibility.  Additional filters and lenses are available at the CFC for a custom configuration. Contact René Brons for further information

5 Analysis stations

5_Analysis_stations

Equipment

Current Team Members :

René Brons BS  

Function : Technician

Phone : +352 26970-308

Fax : +352 26970-397

Email : rene.brons@crp-sante.lu