EVENTS
Biomedical applications of 2DC-MS/MS proteomic approach
26/11/2010 10:00 am
- Speaker: Dr. Pier Luigi Mauri
- Language: English
- Location: BAM - Mc Clintock room
- Contact: bruno.domon@crp-sante.lu
Dr. Mauri has experience in the development and application of several technologies (HPLC, capillary electrophoresis, mass spectrometry) for the identification and quantification of several biomolecules in complex matrices. He has been involved in the study of the bioavailabily of several drugs in humans and animals.
Recently, h e has set-up a proteomics laboratory based the MudPIT methodology (2DC-MS/MS) and a parallel computing system for proteomics. The latter instrumentation allows for the proteomic profiling of biological samples, without limitations, according to to protein molecular weight, pI or hydrophobicity and guarantees high productivity. Using this methodology, secreted proteins from tumor (FASEB 2005) and immune system cells have been characterized. Furthermore, direct analysis of biological samples (JMS 2007), enzymatic complexes (Biochimie 2006), development of novel methodological approaches for studying switch-redox proteins (JBC 2005 and 2006, JMB 2006), and structural and functional characterization of proteins (Biol. Chem. 2004, FEBS 2006) have been performed.