EVENTS
Pathway Proteomics using Protein Microarrays
20/08/2010 9:00 am
- Speaker: Jan van Oostrum - Zeptosens
- Language: English
- Location: Mc Clintock Room - Bam Strassen
- Contact: andre.steinmetz@crp-sante.lu
Molecular signalling pathways are frequently triggered by extracellular molecules binding receptors and activating relay systems inside cells affecting cellular behaviour and fate. For many genetic disorders a link between disease and signalling pathways have been established consequently asystematic analysis of dynamic cellular networks provides an opportunity for pharmaceutical discovery, by taking into consideration the complex biological context of drug targets, rather than observing the targets in isolation. Recently, we implemented and optimized a proteomics platform based on “reverse” protein arrays (RPA) that is particularly suitable to monitor cell signalling events. These arrays are based on the principle that complex protein mixtures or proteomes (such as cell or tissue lysates) are spotted in an array format and probed with selected fluorescent antibodies in a multiplexed manner. To ensure high levels of sensitivity and signal to noise ratio of these RPAs, we are using planar waveguide technology. The advantage of the evanescent field fluorescence detection ensures that only analyte-bound fluorescent antibodies contribute to the signal. This method makes it feasible to obtain quantitative and kinetic protein expression profiles and signaling information in a wide variety biospecimen.