NEWS
2011 - 02 - 08 Focus on publications at Norlux Neuro-Oncology Laboratory
Anna Golebiewska from the Norlux Neuro-Oncology Laboratory has recently published a paper in the highly rated journal Cell Stem Cell (impact factor above 23). “ Critical appraisal of the side population assay in stem cell and cancer stem cell research” By Anna Golebiewska, Nicolaas H.C. Brons, Rolf Bjerkvig and Simone P. Niclou Norlux Neuro-Oncology Laboratory, Oncology Department, and Core Facility Flow Cytometry, LBR2, CRP-Santé, Luxembourg Cell Stem Cell. 2011 Feb 4;8(2):136-47. The work addresses the popular 'Side Population' technology, a surrogate marker for stem cells, often used in stem cell and cancer stem cell research. It is a very sensitive and critical flow cytometric technique that is prone to errors. The publication addresses the pitfalls of the methodology and how to circumvent them.
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The ‘Side Population’ (SP) discrimination assay is a flow cytometry method used to detect stem cells based on the dye efflux properties of ABC transporters. We discuss the SP assay and its applications in stem cell biology, with an emphasis on the technical challenges related to sample preparation, data acquisition, analysis and interpretation. We highlight the value of multicolor phenotyping, the impact of DNA ploidy and the importance of distinguishing graft versus host cells for an appropriate SP discrimination. To improve the consistency and reliability of data between laboratories, we propose a set of recommendations for SP assay data reporting.
The Norlux Neuro-Oncology team has just published another work of major interest by Olivier Keunen and colleagues, shedding light on the physiological consequences of anti-angiogenic treatment in glioblastoma,
The title is:
Anti-VEGF treatment reduces blood supply and increases tumor cell invasion in glioblastoma. Olivier Keunen, Johansson M, Oudin A, Sanzey M, Abdul Rahim SA, Fack F, Thorsen F, Taxt T, Bartos M, Jirik R, Miletic H, Wang J, Stieber D, Stuhr L, Moen I, Brekke Rygh C, Bjerkvig R and Niclou SP. Proc Natl Acad Sci USA. (in press)