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2011 - 06 - 14 Mario Schellenberger defended his PhD thesis at the University of Trier, Germany
Department of Immunology (LNSI). Mario Schellenberger recently defended his Ph.D. thesis (summa cum laude) at the University of Trier, Germany. The title of his PhD thesis is “Immunoprophylactic approach against chemical carcinogenesis” . Mario Schellenberger has written or co-authored 4 publications, another publication is in preparation. He has given numerous oral presentations, both as department seminars and at national and international meetings.
The main objective of his study was to investigate the potential of hapten specific antibodies to protect against chemical carcinogenesis in mice as the basis of an immunoprophylactic approach against carcinogens using Benzo[a]pyrene (B[a]P) as model compound, an environmental pollutant emanating from both natural and anthropogenic sources. High doses of carcinogens conveniently used in in vivo animal models cannot be matched with antibody concentrations obtained by immunisation and environmental relevant exposure to B[a]P do not permit to directly measure of chemical carcinogenesis. Therefore the pharmacokinetic, metabolism and B[a]P mediated immunotoxicity were chosen as experimental read-outs.
During its PhD he developed and carried out many different techniques including molecular biology techniques (qPCR, sequencing, Affymetrix microarray technology, ELISA, Fluorescence Microscopy), proteomic techniques (HPLC, western blotting, Maldi TOF analysis) protein purification techniques (affinity-, size chromatography and ultracentrifugation) and in coupling chemistry.
Mario Schellenberger will stay in our Institute as a post-doctoral scientist and will continue to work on the TOBAVAC 3 project, which so far results in of 11 publications:
1. Evaluation of adjuvants for a candidate conjugate vaccine against benzo[a]pyrene. MT Schellenberger, S Farinelle, S Willième, CP Muller, Human Vaccines, IF 1.94. 7, 166-173, 2011. http://www.landesbioscience.com/journals/vaccines/article/14579/
2. Effects of antibodies induced by a conjugate vaccine, on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone absorptive transport, metabolism and proliferation of human lung cells , SS De Buck, MT Schellenberger , C Ensch, CP Muller, International Journal of Cancer, IF 4.734. 127, 513-20, 2010 . http://www.ncbi.nlm.nih.gov/pubmed/19960439
3. Modulation of Benzo[a]pyrene induced immunotoxicity in mice actively immunized with a B[a]P-diphtheria toxoid conjugate , MT Schellenberger , N Grova, S Willième, S Farinelle, EJF Prodhomme, CP Muller. Toxicology & Applied Pharmacology, IF 3.364, 37-45, 240, 2009, ISSN 1096-0333, http://www.ncbi.nlm.nih.gov/pubmed/19573549
4. Modulation of carcinogen bioavailability by immunisation with benzo[a]pyrene-conjugate vaccines, N Grova, EJ Prodhomme, MT Schellenberger , S Farinelle, CP Muller. Vaccine, IF 3.377, 42-51, 27, 2009, ISSN 1873-2518, http://www.ncbi.nlm.nih.gov/pubmed/19406187
5. Sub-acute administration of benzo[a]pyrene (B[a]P) reduces anxiety-related behaviour in adult mice and modulates regional expression of N-methyl-d-aspartate (NMDA) receptors genes in relevant brain regions , N Grova, H Schroeder, S Farinelle, EJF Prodhomme, A Valley, CP Muller. Chemospere, IF 3.054, S295-302, 73, 2008, ISSN 0045-6535, http://www.ncbi.nlm.nih.gov/pubmed/18442843
6. Modulation of behavior and NMDA-R 1 gene mRNA expression in adult female mice after sub-acute administration of benzo(a)pyrene , N Grova, A Valley, JD Turner, A Morel, CP Muller, H Schroeder. Neurotoxicology, IF 2.409, 630-36, 28, 2007, ISSN 0161-813X, http://www.ncbi.nlm.nih.gov/pubmed/17397927
7. Synthesis of 4-[2-Amino-ethyl(nitrosamino)]-1-pyridin-3-yl-butan-1-one, a new NNK hapten for the induction of N-nitrosamine specific antibodies , EJF Prodhomme, C Ensch, FB Bouche, T Kaminski, S Deroo, P Seck, G Kirsch, CP Muller. Bioconjugate Chemistry, IF 4.584, 2045-2053, 18, 2007, ISSN 1043-1802, http://www.ncbi.nlm.nih.gov/pubmed/17939731
8. Specific antibody modulates absorptive transport and metabolic activation of benzo[a]pyrene across Caco-2 monolayers , SS De Buck, P Augustijns, CP Muller. J Pharmacol Exp Ther, IF 4.309, 640-6, 313, 2005, ISSN 0022-3565, http://www.ncbi.nlm.nih.gov/pubmed/15665140
9. Modulation of metabolism and adverse effects of benzo[a]pyrene by specific antibody: a novel host factor in environmental carcinogenesis? SS De Buck, FB Bouche, A Brandenburger, CP Muller. Carcinogenesis, IF 4.39, 835-44, 26, 2005, ISSN 0143-3334, http://www.ncbi.nlm.nih.gov/pubmed/15637092
10. Immunoprophylactic approaches against chemical carcinogenesis. SS De Buck, CP Muller, Vaccine, IF 3.377, 2403-6, 23, 2005. ISSN 0264-410X, http://www.ncbi.nlm.nih.gov/pubmed/15755636
11. Behaviour modulation and expression of N-methyl D-aspartate receptor genes in adult female mice after a chronic administration of Benzo[a]pyrene, N Grova, H Schroeder, A Valley, JD Turner, CP Muller, Proceedings of the “25th International Symposium on Halogenated Environmental Organic Pollutants and Pops”,Toronto, Dioxin 418-420, 2005.