Confocal Microscopy
Céline Hoffmann
Address: 84 Val Fleuri - 1526 Luxembourg
Phone. (+352) 26 970 253
Fax. (+352) 26 970 390
celine.hoffmann@crp-sante.lu
IMMUNOLABELING AND GFP IMAGING
Proteins of interest can be labeled in fixed cells by immuno-techniques or tagged in live cells with a GFP fusion protein.
Tobacco endoplasmic reticulum
Céline Hoffmann, LBMV
Live observation of tobacco BY2 cells expressing an ER-resident protein (green). The picture is a 3D reconstruction of 33 planes.
Nucleolar localisation
Céline Hoffmann, LBMV
Immuno-localisation of a protein in the nucleolus (green) of tobacco BY2 cells. The nucleus was stained with DAPI (blue). The picture is a single confocal plane.
Actin cytoskeleton
Céline Hoffmann, LBMV
Co-localisation of an actin-binding protein (fimbrin-GFP, green) and actin cytoskeleton (rhodamine-phalloidin, red). Merge picture in yellow.
Co-localisation graph (Co-localisation ratio 0.9)
