Confocal Microscopy
Céline Hoffmann
Address: 84 Val Fleuri - 1526 Luxembourg
Phone. (+352) 26 970 253
Fax. (+352) 26 970 390
celine.hoffmann@crp-sante.lu
OBSERVATION OF CELL PHYSIOLOGICAL STATE
Intracellular pH: Lily pollen tube containing a pH indicator (SNARF-5F, Invitrogen). Observation of the pH gradient and the basic zone at the growing end of the tube.
Céline Hoffmann, LBMV
Intracellular calcium:
calcium accumulation during drug treatment (A23187, Invitrogen) in Arabidopsis cells (Calcium sensor: XRhod-1, Invitrogen)
| T = 0 |
t = 1h |
t = 2h |
t =3h |
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Quantification of fluorescence increasing
