Confocal Microscopy
Céline Hoffmann
Address: 84 Val Fleuri - 1526 Luxembourg
Phone. (+352) 26 970 253
Fax. (+352) 26 970 390
celine.hoffmann@crp-sante.lu
TIME-LAPSE EXPERIMENTS
Live cell experiments allow observation of cells from seconds to several hours. It is possible to observe protein localization under different physiological conditions e.g. drug treatment or stress. After time-lapse acquisition, it is possible to track objects in 2D or in 3D and to measure characteristics such as velocity.
Time-lapse: growing Arabidopsis pollen tube expressing a GFP -Actin binding protein. This is a 10 minutes time-lapse with 1 picture every 30 seconds.
Céline Hoffmann, LBMV
Kymograph: this graph represents a line of the picture plotted as a function of time. A 30 µm line (in yellow) was selected to see the edge of the tube and its progress during 10 minutes.
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Track in 2D: objects shown here are Golgi stacks observed in living BY2 cells (tracking with Metamorph). Time-lapse: 1 picture every 10 seconds, total duration 10 minutes.
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Track of one Golgi stack |
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Mean velocity 0,05 micron/s |
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Track in 3D: swimming algae from Imaris (picture from the software database)
