Confocal Microscopy
Céline Hoffmann
Address: 84 Val Fleuri - 1526 Luxembourg
Phone. (+352) 26 970 253
Fax. (+352) 26 970 390
celine.hoffmann@crp-sante.lu
ANTIBODIES AND FLUORESCENT PROBES AVAILABLE ON THE PLATFORM
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1. Primary antibodies
- Anti-Microtubules made in mouse (ab3194) from abcam
- Anti-Actine made in rabbit from Sigma (A5060)
- Anti-arabidopsis Actine made in mouse from Sigma (A0480)
- Anti-PDI (ER marker) made in mouse from abcam (ab2792),
- Anti-58K Golgi protein made in mouse from abcam (ab6284),
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2. Secondary antibodies
| Goat Anti Mouse (GAM ) |
488 nm (A11029) |
568nm |
|
| Goat Anti Rabbit (GAR) |
488 nm |
568 nm |
|
| Goat Anti- Rat (GARt) |
568 nm |
633 nm |
|
| Donkey Anti Goat (DA G) |
488 nm |
568 nm |
|
| Donkey Anti Sheep (DAS) |
568 nm (A21099) |
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3. Fluorescent Probes
- Nucleus Staining: DAPI, SYTOX-Green, 7-AAD, TO-PRO3
- Actin: Phalloidin A488, A555, A568, Rhodamine
- Plasma membrane: FM 4-64FX
- Golgi apparatus : WGA-A555
- Calcium sensitive dyes: Fluo 4, X-Rhod1
- pH sensitives dyes : SNARF 1F, 4F, 5F
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4. Other products
- Glutaraldhéhydes 8% in ampoule 10x2ml (EMS)
- Formaldhéhyde 20% in ampoule 10x10ml (EMS)
- Image IT FX signal enhancer: block specific background from Alexa conjugared secondary antibodies (Invitrogen, I36933)
- Citifluor: mounting media with antifading (EMS, AF1)
- Prolong anti-fade: mounting medium with antifading (Invitrogen)
- Ascorbic acid, powder
- Cold water fish skin gelatin 40% (EMS),
- Acetylated BSA: Aurion BSAc 10% (EMS)
- Washing buffer: BSAc 0.1% in PBS
- blocking buffer prepared: BB (PBS, BSAc 1%, CWFSG 0,1%, NGS 5%)
- Poly-L-Lysine: from Sigma, MW >300 000 aliquot keep at -20°C, ready to use 1mg/ml.
- Cover-slips: 20x20, 24x50, 22x40, Ø13, Ø16, Ø 25, Ø 42