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Confocal Microscopy

Céline Hoffmann
Address: 84 Val Fleuri - 1526 Luxembourg
Phone. (+352) 26 970 253
Fax. (+352) 26 970 390
celine.hoffmann@crp-sante.lu

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Contact

Confocal Microscopy

Céline Hoffmann
Address: 84 Val Fleuri - 1526 Luxembourg
Phone. (+352) 26 970 253
Fax. (+352) 26 970 390
celine.hoffmann@crp-sante.lu

Find on Google Map

Make a Donation

THE MICROSCOPE

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The microscope is an axiovert 200M from Zeiss

The microscope allows imaging in epifluorescence with a LED illuminating system and a monochromatic camera. Timelapse experiments are also possible with temperature and CO2 control.

  • Illuminating system: Colibri with 3 LED: 365 nm, 470 nm and 555 nm
  • Camera: AxioCam MRm
  • Filters: Set01 (DAPI):
    • BP 365/12 - LP 397 
    • Set09 (YFP):  BP 450-490 - LP 515 
    • Set15 (TRITC): BP 546/12 - LP 590
    • Set38 (FITC): BP 450-490 - BP 500-550
  • Objectives:
Magnification

-----------------------

type

--------------------------

NA

----

contrast

-------------

immersion

-----------------

WD

-------------

10x

Plan NeoFluar

0.3

   

5.6 mm

10x

A-Plan

0.25

PlasDIC

 

4.4 mm

20x

Plan NeoFluar

0.5

Dic II

 

2 mm

20x

LD A-Plan

0.3

PlasDIC

 

4.3 mm

40x

LD Plan NeoFluar

0.6

Phase 2

 

2.9 mm

40x

Plan NeoFluar

1.3

DIC III

oil

0.2 mm

63x

LD Plan NeoFluar

0.75

Phase 2

 

1.7 mm

63x

Plan Apochromat

1.4

DIC III

oil

0.18 mm

63x

C-Apochromat

1.2

DIC III

water

0.24 mm

100x

EC Plan NeoFluar

1.3

Phase 3

oil

0.2 m m

Objectives in bold are mounted on the microscope, the others are available for specific experiments.