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Scope

Our fields of interest range from basic research to contract R&D for the diagnostic and vaccine industry.

  • Immunology and genetic/antigenetic diversity of old and new emerging viruses: relevance for detection, vaccination, pathogenicity, drug resistance, development of new treatment strategies
  • Development of immune-preventive strategies against infectious agents and against environmental risk factors, such as carcinogens.
  • Crosstalk between the immune system and the neuroendocrine system in particular under stress: gene structure, the transcriptional and translational regulation of nuclear receptors, such as the glucocorticoid receptor as the main stress response mediator.
  • Perinatal programming and epigenetics of the immune system.
  • Proteomics studies using 2D DIGE technology paired with MALDI-TOF mass spectrometry and LC-MS in the above areas and other fields in collaboration.

Contact

Department of Immunology
Head of Research Unit: Prof. Dr. Claude P. Muller
Laboratoire National de Santé
20A, rue Auguste Lumière, L-1950 Luxembourg
Tel.: +352 49 06 04 220
Fax: +352 49 06 86
E-mail: claude.muller@crp-sante.lu

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TECHNOLOGIES

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Immunology  

  • T cell and B cell hybridomas  
  • T cell lines monoclonal and polyclonal antibody production  
  • Various formats of immunoassays (ELISA plate readers for absorption, fluorescence, chemiluminsecence)  
  • Flow cytometry including cell sorting and automated cell cloning  
  • Phenotyping of clinical samples  
  • Pepscan (see also Chemistry)  
  • Cytokine expression (ELISA, mRNA by real-time PCR)  
  • Breeding of specific pathogen-free mice  
  • In vivo and in vitro immunogencity studies  

Virology  

  • BSL2 and BSL3 laboratories  
  • Virus isolation  
  • Production and purification of viruses  
  • Serological tests  
  • Seroprevalence studies  
  • Genotyping of viruses  
  • Phylogenetic analyzes  
  • Molecular epidemiology  
  • Epidemic surveillance  
  • Diagnostic and sequencing PCR for respiratory viruses, animal viruses, poultry viruses (avian influenza)  
  • Hepatitis viruses, rubella virus, measles virus and other fever rash viruses; tick borne viruses  

Molecular Biology

  • PCR and RT-PCR  
  • Real-time PCR  
  • Restriction ligation cloning  
  • Promoter less vectors for promoter analysis  
  • IRES vectors for co-expression of target sequences  
  • Gateway cloning  
  • GFP tag  
  • GST tag  
  • 6xHis tag  
  • Lumio (red) tag  
  • Tet-On system  

Transfection

  • Cationic lipid technique  
  • Electroporation  
  • Lentiviral infection  

Protein-protein interactions

  • Yeast two hybrid system  

DNA-protein interactions

  • Capillary EMSA  
  • Gel shift assays  
  • DNAse protection assay  

Sequencing

  • Sanger sequencing  
  • CpG methylation (bisulphite) sequencing  

Recombinant protein production and purification

  • PEWA, 6x his tag purification  

Eucaryote and procaryote expression systems

  • Semliki Forest Virus expression system  
  • E coli  
  • Baculovirus  
  • Random phage display peptide libraries
  • Analytical and preparative HPLC for DNA and proteins

 Chemistry  

  • Peptide synthesis and purification  
  • HPLC and Mass spectrometry (see platforms)  
  • Chemical derivatisation of haptens  
  • Conjugation chemistry  

   Technology Platforms